FOXO4 10vials/box

Product Basic Information

Full Name: FOXO4-DRI (D-Retro-Inverso Peptide, also known as Proxofim)

CAS Number: 2460055-10-9

Nature: A D-type reverse-synthetic polypeptide composed of 46 amino acids (all D-amino acids + sequence inversion), possessing resistance to protease degradation, high stability, and cell penetration.

Form: Sterile lyophilized powder, individually packaged in vials.

Uses: Senescent cell serolysis, anti-aging, tissue function repair, in vitro research reagent for age-related diseases.

II. Mechanism of Action

Targeting the interaction between FOXO4 and p53 proteins, it achieves selective serolysis of senescent cells. The core pathway is as follows:

Target Specificity: Senescent cells highly express FOXO4, which binds to and impounds p53 in the cell nucleus, preventing p53 from initiating apoptosis, maintaining the survival of senescent cells, and secreting SASP inflammatory factors (IL-6, TNF-α, etc.).

Competitive Blocking: After entering cells, FOXO4-DRI binds with high affinity to the TAD2 domain of p53, competitively disrupting the FOXO4-p53 complex and releasing p53.

Selective Apoptosis: Free p53 translocates to mitochondria, activating pro-apoptotic proteins such as BAX, inducing apoptosis only in senescent cells, with no significant effect on healthy proliferating cells.

Structural Advantages: D-type amino acid modification makes it resistant to in vivo protease degradation, resulting in a longer half-life and higher bioavailability.

III. Core Scientific Efficacy

Senescent Cell Clearance: Efficiently clears senescent fibroblasts, endothelial cells, and testicular interstitial cells in in vitro models, reducing SA-β-gal positivity and SASP factor secretion.

Tissue Function Repair: Improves vascular endothelial aging, reduces vascular inflammation, and restores endothelium-dependent vasodilation, suitable for research on the mechanisms of atherosclerosis and hypertension.

Clears senescent testicular interstitial cells, restoring testosterone synthesis capacity, useful in in vitro models of male late-onset hypogonadism.

Reduces senescent cartilage cells, alleviates joint inflammation, and is suitable for osteoarthritis-related experiments.

Anti-aging and metabolic regulation: Improves the tissue microenvironment and enhances metabolic efficiency after clearing senescent cells, making it suitable for metabolic syndrome and skin aging research.

Pathological scar intervention: Induces apoptosis of senescent fibroblasts in keloids and inhibits SASP, used for exploring the in vitro mechanisms of pathological scars.

Combined use studies: Often used in combination with BPC-157, Epitalon, and NAD+ precursors to conduct multi-target anti-aging and tissue repair combined experiments.

IV. Reconstitution and Storage Guidelines

Reconstitution solvent: Reconstitute the lyophilized powder with sterile water for injection or 0.9% physiological saline, mix gently, and avoid vigorous shaking.

Storage requirements

Unopened: Refrigerate at 2-8℃ protected from light. Do not freeze to prevent damage to the peptide spatial structure.

After reconstitution: Store at 4℃ protected from light.